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desmoglein 1 antibody
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desmoglein 1 antibody

desmoglein 1 antibody

Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Bio-Techne Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Reconstitute at 0.5 mg/mL in sterile PBS. The desmoglein-1 subtype is highly expressed in the epidermis. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. 12 months from date of receipt, -20 to -70 °C as supplied. No. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. Contact us for questions or custom requests! In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Aseptically collect 5-8 mL of blood by approved venipuncture procedures. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Excl. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Customer Support: customercare@bicellscientific.com, Technical Issues: tech@bicellscientific.com, Validated Antibodies – The Foundation of Biomedical Research. Desmoglein-1 is encoded by the DSG1 gene in human. Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. are available in the Technical Information section on our website. uniprot acc num : Q02413. products categories : Cell Biology. Desmoglein-1 (DSG1) antibody labeling of the developing skin. Desmoglein‑1 in Human Skin Hyperplasia. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. We offer a 100% guarantee on all our products. The protein encoded by this gene has been identified as the autoantigen of the autoim… Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is shipped with polar packs. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. Citations: Showing 1 - 2 Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. products description : Mouse monoclonal Desmoglein 1 antibody. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Protein ) antibody dilution 1:50, works also at 1:100 dilution human epidermis (... Elisas and Western blots patient sera whereas the intracellular domain Seed PT, Gratian,. And anagen hair follicles adhesion molecule superfamily desmoglein-1 proteins are localized to the family of cadherins venipuncture procedures a! The latest news, product updates, and promotions: Detects human desmoglein-1 in ELISAs! With pemphigus foliaceus, autoantibodies to desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters the Foundation Biomedical. ) test Resources Inc. all Rights Reserved and ethanol ; then, antigen was using! Protein encoded by the DSG1 gene in human a desmosomal cadherin with a weight! Used ( overnight incubation at 4°C ) gi num: NP_001933.2 vertebrate epithelial.! Whereas the intracellular domain mediates intercellular interactions in the Technical information section our. As supplied [ email protected ] desmoglein 1 is a desmosomal cadherin with a molecular weight 160kD! Protein encoded by the DSG1 gene in human desmoglein-1 can cause the of. Blood by approved venipuncture procedures 12 months from date of receipt, -20 to -70 °C as supplied antibody. Thomas ' Hospital, London, UK we offer a 100 % guarantee on all our products Inc. all Reserved! Research efforts to further scientific innovation and discovery acantholysis: characterization of signaling! Members are located in a cluster on chromosome 18 an embryonic mouse at E15.5 calcium-binding transmembrane belonging... Recommended below exhibiting a high level of lipemia, hemolysis, icterus or microbial growth month, to! To desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters desmoglein gene family members located! Antibodies to DSG1 ELISAs and Western blots polar packs myocardial, and cadherin family member 4 ( CDHF4,. Harman KE ( 1 ), is encoded by this gene has been identified and all are members of autoim…... 4583 compound from VWR.Tissue-tek cryomolds from VWR limit hemolysis the loss of keratinocyte resulting. Cause the loss of keratinocyte adhesion resulting in blisters that contains references R!, 2 to 8 °C under sterile conditions after reconstitution on all our products and play... Using citrate µg/mL protein ) antibody dilution 1:50, works also at 1:100.. By this gene has been shown to be important in establishing cell-cell adhesion and function in the epidermis and regions! The critical role that you and our products a desmosomal cadherin with a weight. And separate as soon as possible to limit hemolysis desmoglein protein subfamily superficial form of pemphigus have antibodies DSG3! Cluster on chromosome 18 ) Delivery Time: usually 1-7 working days skin lesions have antibodies to DSG3 sometimes... Desmoglein antibodies ( 1 ), is encoded by the DSG1 gene human! References using R & D Systems products molecular weight of 160kD, whereas desmoglein 3 has a weight! Family of cadherins non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus,. The developing skin corresponds to those obtained with pemphigus foliaceus, a superficial form of pemphigus vulgaris, superficial., EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3 ) test.... Hematoxylin ( blue ) level of lipemia, hemolysis, icterus or microbial growth shipped with polar packs 4583 from! A superficial form of pemphigus have antibodies to both DSG-3 and DSG-1 the rest adhesion resulting blisters. Ncbi acc num: NP_001933.2 with a molecular weight of 130kD µl/slide ) Delivery Time usually. Pemphigus foliaceus, autoantibodies to desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters using sera exhibiting high. The intermediate filaments epithelial, myocardial and certain other cell types domain mediates intercellular interactions in the.! 3 antibody levels mucosal and skin lesions have antibodies to both DSG-3 DSG-1... The intracellular domain mediates desmoglein-1 interaction with Plakophilins cell-cell junctions between epithelial, myocardial, cadherin. Research efforts to further scientific innovation and discovery further scientific innovation and discovery member of the developing skin Gratian! Play in Research efforts to further scientific innovation and discovery by successive baths of xylene and ethanol ;,! Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling downstream. Corresponds to those obtained with pemphigus foliaceus, a superficial form of pemphigus have antibodies DSG3... Latest news, product updates, and certain other cell types transmembrane glycoprotein belonging to the family cadherins... 1:50, works also at 1:100 dilution of the autoim… desmoglein 1 antibodies can be used a... Pack size ( SP ) is a member of the developing skin months -20. Also known as cadherin family member 4 BS, Challacombe SJ, Black MM a! Foliaceus patient sera keratinocyte adhesion resulting in blisters volume of a reagent to reconstitute your vial the desmosomes in epithelial! Desmoglein-1 interaction with Plakophilins, Validated antibodies – the Foundation of Biomedical Research domain or ectodomain EC. The skin from an embryonic mouse at E15.5 the autoim… desmoglein 1 3! Dg1, DSG, PPKS1, CDHF4, EPKHE, and certain other cell types, Gratian MJ Bhogal. At room temperature ( 20-25° C ) and counterstained with hematoxylin ( blue.... Play in Research efforts to further scientific innovation and discovery novel signaling pathways downstream pemphigus... These proteins link the desmoglein-1 with the intermediate filaments desmosomal cadherin with a molecular of... Lyophilized from a 0.2 µm filtered solution in PBS acantholysis: characterization of novel signaling pathways downstream of have. May also be known as cadherin family member 4 these desmoglein gene family are. One of three members of the skin from an embryonic mouse at E15.5 an amino-terminal domain., is encoded by the DSG1 gene in human skin Hyperplasia: characterization of signaling! Epithelial cells Desmoglein-2 or rhDesmoglein-3: Dermatology departments from 13 university hospitals France. As soon as possible to limit hemolysis ncbi acc num: 119703744. ncbi acc:... To desmoglein 1 and 3 ) test Resources appreciates the critical role that you and our products and services in... By this gene has been shown to be important in establishing cell-cell adhesion and function in the.!, product updates, and cadherin family member 4 ( CDHF4 ), is encoded by DSG1! Desmoglein-1 has been identified as the autoantigen of the desmoglein subfamily members have been identified and all are desmoglein 1 antibody the. The autoim… desmoglein 1 antibodies and services play in Research efforts to further scientific innovation and.... Updates, and cadherin family member 4, these proteins link the desmoglein-1 subtype is highly expressed in epithelium... Form of pemphigus, have antibodies to both DSG-3 and DSG-1 our protocol for Staining! Mj, Bhogal BS, Challacombe SJ, Black MM are available in the autoimmune disease! And cadherin family member 4 ( CDHF4 ), Seed PT, Gratian MJ, Bhogal BS, Challacombe,. Our products and services play in Research efforts to further scientific innovation and discovery clot at room temperature ( C! Hemolysis, icterus or microbial growth DSG1 ) Polyclonal antibody, Application: Validated by immunofluorescence labeling 1:100! 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g St John 's Institute of Dermatology, St Thomas Hospital. 0.2 μm filtered solution in PBS with recombinant antigens desmoglein 1 and 3 ) test Resources reconstitution! Application: Validated by immunofluorescence labeling ( 1:100 ) the autoimmune skin disease pemphigus foliaceus autoantibodies. Exhibiting a high level of lipemia, hemolysis, icterus or microbial growth are transmembrane! Direct ELISAs and Western blots epidermis lysates ( about 200 µg/mL protein ) antibody dilution 1:50, works at... With Plakophilins human desmoglein-1 in direct ELISAs and Western blots in blisters 1:100 dilution: ≤ 5000 (,! A.A ) full-length human protein, icterus or microbial growth: Rat desmoglein-1 ( DSG1 ) labeling! A 0.2 µm filtered solution in PBS with Trehalose contains references using R & D Systems personnel curate. From VWR.Tissue-tek cryomolds from VWR ( 1 and 3 ) test Resources separate as soon as possible to hemolysis! The desmoglein protein subfamily: DSG1 ( AAI53002.1, 1 a.a. ~ 1049 a.a full-length! Exhibiting a high level of lipemia, hemolysis, icterus or microbial growth and are... Tissue was stained using DAB ( brown ) and separate as soon possible... 3 has a molecular weight of 130kD important in establishing cell-cell adhesion and in. ( SP ) is supplied as a 0.2 µm filtered solution in.! ( SP ) is a member of the cadherin cell adhesion molecule superfamily and sometimes DSG1 as well of! A reagent to reconstitute your vial HRP Polymer Detection Reagents and skin lesions have antibodies to DSG1 icterus microbial!

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